Relationship between induction of insulin resistance and selenoprotein expression in skeletal muscle in diabetic mouse model

<p>Selenium supplementation has been investigated as a potential treatment against the development of diabetes. However, recent epidemiological and clinical studies have shown that the prevalence of type 2 diabetes is high in populations with high serum selenium levels. Se may affect glucose metabolism and lipid metabolism through the expression of selenoproteins such as selenoprotein P (SelP) and glutathione peroxidase (GPx). In addition, it has been pointed out that the development of sarcopenia with age-related loss of skeletal muscle mass is associated with insulin resistance. In this study, we analyzed the effect of administration of seleno-L-methionine (SeMet) on the induction of insulin resistance and the expression of selenium protein in skeletal muscle in NSY mice, which are an animal model of human type 2 diabetes characterized by lower insulin secretion due to aging. NSY mice were fed a nomal diet (ND) or a high fat diet (HFD) and administered SeMet. Significant increase in oral glucose tolerance-tested (OGTT), insulin tolerance-tested (ITT) were observed in HFD group. The expression level of GPx1 mRNA in the gastrocnemius muscle was significantly increased in the HFD group regardless of the SeMet intake. In addition, SelP, SelW and GPx1 mRNA expression levels in soleus muscle were significantly increased in the HFD intake group regardless of SeMet intake, and GPx1 mRNA expression levels were significantly increased by SeMet intake. These results suggest that the expression levels of SelP, SelW and GPx1 mRNA in soleus muscle are associated with the development of insulin resistance by ingestion of HFD in NSY mice.</p>