Simultaneous Measurement of Change in Intracellular Calcium Ions and Degranulation in Single Intact Mast Cells from Rat Peritoneum

DOI 15 References
  • Kusumoto Akihide
    Faculty of Pharmaceutical Sciences, Kyushu University
  • Umeoka Ken-ichi
    Faculty of Pharmaceutical Sciences, Kyushu University
  • Toda Shoichi
    Faculty of Pharmaceutical Sciences, Kyushu University
  • Hirashima Naohide
    Faculty of Pharmaceutical Sciences, Kyushu University Faculty of Pharmaceutical Sciences, The University of Tokyo
  • Kirino Yutaka
    Faculty of Pharmaceutical Sciences, Kyushu University Faculty of Pharmaceutical Sciences, The University of Tokyo

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Description

To investigate the relationship between intracellular calcium dynamics and degranulation in single intact mast cells, we developed a method for the simultaneous observation of these two events. Rat peritoneal mast cells were doubly stained with two types of fluorescent dyes, quinacrine and Ca-indicator fluo-3. Basic quinacrine was specifically incorporated in secretory granules and the fluorescence intensity was decreased upon degranulation. With emission at 520 nm, the excitation spectra for the two dyes differed so that alternate excitation at 420 and 490 nm made it feasible to measure intracellular Ca2+ elevation and degranulation, with the time-resolution of 2 s. Degranulation was always accompanied by a preceding transient rise in intracellular Ca2+ yet an increase in intracellular Ca2+ did not necessarily induce degranulation. These findings suggest that intracellular Ca2+ elevation is a necessary but not sufficient condition for degranulation in intact mast cells.

Journal

  • bioimages

    bioimages 3 (1), 1-5, 1995

    Bioimaging Society

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Details 詳細情報について

  • CRID
    1390574732433966080
  • NII Article ID
    10002037427
  • NII Book ID
    AA11084187
  • DOI
    10.11169/bioimages.3.1
  • ISSN
    09192719
  • Text Lang
    en
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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