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Simultaneous Measurement of Change in Intracellular Calcium Ions and Degranulation in Single Intact Mast Cells from Rat Peritoneum
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- Kusumoto Akihide
- Faculty of Pharmaceutical Sciences, Kyushu University
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- Umeoka Ken-ichi
- Faculty of Pharmaceutical Sciences, Kyushu University
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- Toda Shoichi
- Faculty of Pharmaceutical Sciences, Kyushu University
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- Hirashima Naohide
- Faculty of Pharmaceutical Sciences, Kyushu University Faculty of Pharmaceutical Sciences, The University of Tokyo
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- Kirino Yutaka
- Faculty of Pharmaceutical Sciences, Kyushu University Faculty of Pharmaceutical Sciences, The University of Tokyo
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Description
To investigate the relationship between intracellular calcium dynamics and degranulation in single intact mast cells, we developed a method for the simultaneous observation of these two events. Rat peritoneal mast cells were doubly stained with two types of fluorescent dyes, quinacrine and Ca-indicator fluo-3. Basic quinacrine was specifically incorporated in secretory granules and the fluorescence intensity was decreased upon degranulation. With emission at 520 nm, the excitation spectra for the two dyes differed so that alternate excitation at 420 and 490 nm made it feasible to measure intracellular Ca2+ elevation and degranulation, with the time-resolution of 2 s. Degranulation was always accompanied by a preceding transient rise in intracellular Ca2+ yet an increase in intracellular Ca2+ did not necessarily induce degranulation. These findings suggest that intracellular Ca2+ elevation is a necessary but not sufficient condition for degranulation in intact mast cells.
Journal
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- bioimages
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bioimages 3 (1), 1-5, 1995
Bioimaging Society
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Details 詳細情報について
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- CRID
- 1390574732433966080
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- NII Article ID
- 10002037427
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- NII Book ID
- AA11084187
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- ISSN
- 09192719
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- Text Lang
- en
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed