Simultaneous Measurement of Change in Intracellular Calcium Ions and Degranulation in Single Intact Mast Cells from Rat Peritoneum

Kusumoto Akihide, Umeoka Ken-ichi, Toda Shoichi, Hirashima Naohide, Kirino Yutaka

doi:10.11169/bioimages.3.1

Simultaneous Measurement of Change in Intracellular Calcium Ions and Degranulation in Single Intact Mast Cells from Rat Peritoneum

DOI 参考文献15件

Kusumoto Akihide

Faculty of Pharmaceutical Sciences, Kyushu University
Umeoka Ken-ichi

Faculty of Pharmaceutical Sciences, Kyushu University
Toda Shoichi

Faculty of Pharmaceutical Sciences, Kyushu University
Hirashima Naohide

Faculty of Pharmaceutical Sciences, Kyushu University Faculty of Pharmaceutical Sciences, The University of Tokyo
Kirino Yutaka

Faculty of Pharmaceutical Sciences, Kyushu University Faculty of Pharmaceutical Sciences, The University of Tokyo

この論文をさがす

CiNii Books

説明

To investigate the relationship between intracellular calcium dynamics and degranulation in single intact mast cells, we developed a method for the simultaneous observation of these two events. Rat peritoneal mast cells were doubly stained with two types of fluorescent dyes, quinacrine and Ca-indicator fluo-3. Basic quinacrine was specifically incorporated in secretory granules and the fluorescence intensity was decreased upon degranulation. With emission at 520 nm, the excitation spectra for the two dyes differed so that alternate excitation at 420 and 490 nm made it feasible to measure intracellular Ca²⁺ elevation and degranulation, with the time-resolution of 2 s. Degranulation was always accompanied by a preceding transient rise in intracellular Ca²⁺ yet an increase in intracellular Ca²⁺ did not necessarily induce degranulation. These findings suggest that intracellular Ca²⁺ elevation is a necessary but not sufficient condition for degranulation in intact mast cells.