Ultrasound irradiation enhances the inhibitory effect of short chain fatty acids on profibrotic factor expressions

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  • 短鎖脂肪酸の線維化促進因子発現抑制に対する超音波照射の促進作用
  • タンサ シボウサン ノ センイカ ソクシン インシ ハツゲン ヨクセイ ニ タイスル チョウオンパ ショウシャ ノ ソクシン サヨウ

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Abstract

<p>Sonoporation of molecule delivery into the cell by ultrasound has been used for gene inductions. We reported the antifibrogenic effects of short chain fatty acids which regulate gene transcription via acetylation of histone in the nucleus. In the present study, we investigated the effects of ultrasound (US) irradiation on antifibrogenic action by butyrate of short chain fatty acids. Ear lobe keloid fibroblasts were treated with butyrate (4 or 16 mM) for 48 h. US irradiations were conducted at 0 h and 24 h after butyrate administration. Pulse (20%) and continuous mode US of 0.5 W/cm2 was applied. mRNA expression of transforming growth factor-β1 (TGF-β1) and a-smooth muscle actin (a-SMA) were evaluated after butyrate treatments. Whereas butyrate of 4 mM did not inhibit TGF-β1 and a-SMA expressions, butyrate combined with pulse or continuous mode US significantly inhibited TGF-β1 (p＜0.01). Butyrate of 16 mM strongly inhibited TGF-β1 and a-SMA expression, and a combination of butyrate and US did not promote those effects. These results suggest promotional effects of US irradiation on the physiological action of butyrate.</p>

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