Calorimetric Analysis of the Growth of Anaerobic Microbes Cultured on Insoluble Carbon Sources
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- Aoki Naoko
- 三重大学
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- 三宅 英雄
- 三重大学
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Methods for the measurement of anaerobic microbial growth are generally complex. Furthermore, aggregation and precipitation make it difficult to estimate bacterial cell counts using turbidity. Recently, we demonstrated that heat evolution is a good indicator of the growth of anaerobic bacteria cultured on soluble carbon sources. In this study, we validated the use of calorimetry to measure growth on insoluble carbon sources using Clostridium spp. When Clostridium cellulovorans and C. thermocellum were cultured on crystalline cellulose, their optical density values over time were irregular. However, the thermograms indicating the heat evolution and the integrated ATP concentrations of the cultures showed highly correlated growth curves. When C. acetobutylicum and C. beijerinckii were cultured on corn starch, peaks were observed at the initial stage based on thermograms and ATP concentration measurements. Thin-layer chromatography revealed that these peaks corresponded to the rapid metabolism of monosaccharides and disaccharides in the culture broth. Thus, the growth of anaerobic microbes cultured on insoluble carbon sources can be measured conveniently and accurately by calorimetry, without confounding effects of aggregation or precipitation. Growth can be measured with high sensitivity, comparable to that of ATP measurements, indicating that calorimetry is a promising method for measuring microbial growth.
収録刊行物
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- 熱測定
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熱測定 45 (2), 70-72, 2018-04-25
日本熱測定学会
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詳細情報 詳細情報について
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- CRID
- 1390575661579023488
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- NII論文ID
- 40021609635
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- NII書誌ID
- AN00199597
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- ISSN
- 18841899
- 03862615
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- NDL書誌ID
- 029127019
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可