書誌事項
- タイトル別名
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- Establishment of a novel method for analysis of biological functions of myeloid cell subpopulation in cardiovascular diseases using TRECK system.
抄録
<p>［Background］Though the importance of myeloid cells in the cardiac remodeling after myocardial infarction (MI) is widely accepted, it remains to be fully elucidated how myeloid cells regulate post-infarct inflammation, at least partially, because subpopulation-specific cell knock-out methods are not available.</p><p>［Methods and Results］We generated transgenic mice expressing diphtheria toxin receptor (DTR)/GFP fusion protein under the control of CD11b promoter in a Cre recombinase-expressing cell-specific manner (CD11b-DTR TG mice). Double TG mice (DTG mice) were generated by crossing CD11b-DTR TG mice with LysM-Cre mice that express Cre recombinase preferentially in monocytes/macrophages. The MI model was created in DTG mice by ligation of the left anterior descending branch. Flow cytometry analysis revealed that monocytes were labeled with GFP in the peripheral blood 4 days after MI. Consistently, immunofluorescent microscopic analysis showed that GFP⁺ cells infiltrated into the infarcted heart. Importantly, the administration of diphtheria toxin resulted in the depletion of GFP⁺ cells in peripheral blood and post-infarct myocardium. </p><p>［Conclusion］CD11b-DTR TG mice are useful for labeling and/or depleting subpopulation of myeloid cells in MI model.</p>
収録刊行物
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- 日本薬理学会年会要旨集
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日本薬理学会年会要旨集 96 (0), 3-B-O09-3-, 2022
公益社団法人 日本薬理学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390576037462470912
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- ISSN
- 24354953
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
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- 抄録ライセンスフラグ
- 使用不可