Rapid derivatization of phosphorus-containing amino acid herbicides in plasma and urine using microwave heating

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  • Mohri Hiroyuki
    Forensic Science Laboratory, Fukuoka Prefectural Police H.Q. Graduate School of Pharmaceutical Sciences, Kyushu University
  • Mohri Kazuko
    Forensic Science Laboratory, Fukuoka Prefectural Police H.Q.
  • Gohda Akinaga
    Forensic Science Laboratory, Fukuoka Prefectural Police H.Q.
  • Ishii Yuji
    Graduate School of Pharmaceutical Sciences, Kyushu University

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<p>We developed a derivatization technique that involves microwave heating to reduce the overall forensic analysis time of phosphorus-containing amino acid herbicides (PAAHs). Combined with an extraction method that uses titanium (IV) oxide (TiO2), we were able to obtain a practical analytical method for PAAHs and their metabolites in samples intended for poisoning cases. The optimized derivatization conditions were 700 W power and 5-min irradiation time, which is a significant time-saving. The plasma samples extracted using TiO2-packed Tip columns and derivatized under the optimized conditions had an intra-day accuracy and precision within 9.3% and 9.0%, respectively. The intermediate accuracy and precision were within 8.8% and 8.5%, respectively, and the recoveries were more than 91.2%. Similarly, for urine samples, the intra-day accuracy and precision were within 13.3% and 9.1%, respectively. The intermediate accuracy and precision were within 13.6% and 10.3%, respectively, and finally, the recoveries were more than 88.2%. In addition to reducing the pretreatment time, this method was suitable for reducing the overall labor burden on laboratories responsible for routine analysis because of its stable validation data.</p>

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