Evaluation of Legionella pneumophila SGUT Serotypes Isolated from Bath Water Using a Multiplex-PCR Serotyping Assay
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- Komatsu Shoko
- Department of Infectious Diseases, Kobe Institute of Health, Japan
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- Tanaka Shinobu
- Department of Infectious Diseases, Kobe Institute of Health, Japan
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- Nakanishi Noriko
- Department of Infectious Diseases, Kobe Institute of Health, Japan
書誌事項
- タイトル別名
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- Evaluation of <i>Legionella pneumophila</i> SGUT Serotypes Isolated from Bath Water Using a Multiplex-PCR Serotyping Assay
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<p>Legionella pneumophila, the primary causative agent of Legionnaires’ disease, is classified into at least 15 serogroups (SGs). Before genotyping, serotyping is first performed to limit the sources of L. pneumophila infections that caused an outbreak. In addition to conventional assays using monoclonal or polyclonal antisera, serotyping using multiplex polymerase chain reaction (M-PCR) was recently developed for L. pneumophila. In this study, we applied the M-PCR system to 41 strains that remained to be SGUT (untypable) by slide agglutination tests among the 220 L. pneumophila strains isolated from bath water in Kobe City during 2016–2020, to determine SG-genotypes (SGg) by PCR amplification of the specific target gene of the SGs. Among the 41 SGUT strains, SGg4/10/14 was the most predominant (24/41, 58.5%), followed by SGg1 (7/41, 17.1%). Seven strains, except for the strains determined as SGg1, were identified as belonging to a single SGg by M-PCR serotyping (SGg5 [3/41, 7.3%], SGg8 [3/41, 7.3%], and SGg7 [1/41, 2.4%]). Furthermore, we found that the seven strains identified as SGg1 harbored particular genotypes. In conclusion, the M-PCR serotyping assay will be helpful for investigating the distribution of L. pneumophila in environmental and clinical settings.</p>
収録刊行物
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- Japanese Journal of Infectious Diseases
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Japanese Journal of Infectious Diseases 76 (1), 77-79, 2023-01-31
国立感染症研究所
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詳細情報 詳細情報について
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- CRID
- 1390576347186953728
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- NII書誌ID
- AA1132885X
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- ISSN
- 18842836
- 13446304
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- NDL書誌ID
- 032637860
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- PubMed
- 36047173
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可