Identification, characterization and expression of a glutamic protease gene from <i>Ganoderma lucidum</i>

DOI
  • KUMAKURA Kei
    Department of Applied Biological Science, Faculty of Agriculture, Takasaki University of Health and Welfare, 54, Nakaorui, Takasaki, Gunma 370-0033, Japan
  • OKAMOTO Kengo
    Department of Applied Biological Science, Faculty of Agriculture, Takasaki University of Health and Welfare, 54, Nakaorui, Takasaki, Gunma 370-0033, Japan
  • KOBAYASHI Taito
    Department of Applied Biological Science, Faculty of Agriculture, Takasaki University of Health and Welfare, 54, Nakaorui, Takasaki, Gunma 370-0033, Japan
  • NAGAI Toshitada
    Department of Applied Biological Science, Faculty of Agriculture, Takasaki University of Health and Welfare, 54, Nakaorui, Takasaki, Gunma 370-0033, Japan
  • MATSUOKA Hiroki
    Department of Applied Biological Science, Faculty of Agriculture, Takasaki University of Health and Welfare, 54, Nakaorui, Takasaki, Gunma 370-0033, Japan

Bibliographic Information

Other Title
  • マンネンタケにおけるグルタミン酸プロテアーゼ遺伝子の同定と発現解析

Abstract

We identified and characterized a glutamic acid protease gene (GlGLP1) from Ganoderma lucidum. The gene consisted of three exons with a total length of 807 bp containing the start and stop codons. It encoded a 268 amino acid protein containing a signal peptide. The expression level of GlGLP1 changed during growth and after harvesting, and was increased in the fruiting body primordia in multiple strains. These results suggest that GlGLP1 is involved in processes from mycelium to fruiting body primordium formation. Furthermore, the expression was confirmed by separating the pileus and stipe of the fruiting body. High expression of GlGLP1 was confirmed in the pileus part approaching maturity. GlGLP1 was also considered to be involved in pileus maturation and sporulation.

Journal

Details 詳細情報について

  • CRID
    1390576424448403328
  • DOI
    10.24465/msb.29.4_154
  • ISSN
    24327069
    13487388
  • Text Lang
    ja
  • Data Source
    • JaLC
  • Abstract License Flag
    Allowed

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