Effects of Coarse and Fine Atmospheric Particulate Matter on a Mast Cell Line

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  • Kataoka Hiromi
    Hygiene Chemistry Laboratory, Department of Pharmacy, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women’s University
  • Tanaka Kaori
    Hygiene Chemistry Laboratory, Department of Pharmacy, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women’s University
  • Tazuya-Murayama Keiko
    Center for Advancing Pharmaceutical Education, Daiichi University of Pharmacy
  • Yamashita Taku
    Hygiene Chemistry Laboratory, Department of Pharmacy, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women’s University
  • Nishikawa Jun-ichi
    Hygiene Chemistry Laboratory, Department of Pharmacy, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women’s University

Bibliographic Information

Other Title
  • 粗大及び微小大気粒子状物質がマスト細胞株に及ぼす影響
  • ソダイ オヨビ ビショウ タイキ リュウシジョウ ブッシツ ガ マスト サイボウカブ ニ オヨボス エイキョウ

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Abstract

<p>We investigated the cytotoxicity on a mast cell line (C57 cells) of water-soluble extracts of coarse (>3 µm, PM>3) and fine (0.05–3 µm, PM0.05–3) atmospheric particulates collected from April 2016 to March 2019 in Fukuoka, Japan. We examined the direct cytotoxicity with punched-out membrane filter fragments of PM>3 and PM0.05–3 collected from April 2019 to March 2021, without extraction of the components. Also, cell proliferation and degranulation assays were conducted under conditions which caused no cytotoxicity with water-soluble extracts of PM>3 from FY2016 and PM>3 direct samples from FY2019. The findings revealed the significant direct cytotoxicity of many PM>3 and all PM0.05–3 samples, with higher cytotoxicity for PM0.05–3 (FY2019–2020). These results were different from the cytotoxicity effects of water-soluble extracts of PM>3 and PM0.05–3 samples (FY2016) in previous studies. In addition, inhibition of cell proliferation and induction of degranulation were significantly induced in a few PM>3 samples, showing a correlation with the suspended particulate matter (SPM) concentrations. This method using punched-out membrane filters is convenient and useful for assessing the direct effects of atmospheric particles on a small scale.</p>

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 143 (2), 159-170, 2023-02-01

    The Pharmaceutical Society of Japan

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