Sasa veitchii extracts protect phenytoin-induced cell proliferation inhibition in human lip mesenchymal cells through modulation of miR-27b-5p
-
- TSUKIBOSHI Yosuke
- Department of Pharmacy, Gifu University of Medical Science
-
- OGATA Aya
- Department of Pharmacy, Gifu University of Medical Science
-
- NOGUCHI Azumi
- Department of Pharmacy, Gifu University of Medical Science
-
- MIKAMI Yurie
- Section of Oral and Maxillofacial Oncology, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University
-
- YOKOTA Satoshi
- Division of Cellular and Molecular Toxicology, Center for Biological Safety and Research, National Institute of Health Sciences
-
- OGATA Kenichi
- Section of Oral and Maxillofacial Oncology, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University
-
- YOSHIOKA Hiroki
- Department of Pharmacy, Gifu University of Medical Science
書誌事項
- タイトル別名
-
- <i>Sasa veitchii</i> extracts protect phenytoin-induced cell proliferation inhibition in human lip mesenchymal cells through modulation of <i>miR-27b-5p </i>
この論文をさがす
説明
<p>A cleft lip, with or without a cleft palate, is a common birth defect caused by environmental factors or genetic mutations. Environmental factors, such as pharmaceutical exposure in pregnant women, are known to induce cleft lip, with or without cleft palate in the child. This study aimed to investigate the protective effect of Sasa veitchii extract (SE) on phenytoin-induced inhibition of cell proliferation in human lip mesenchymal cells (KD cells) and human embryonic palatal mesenchymal cells (HEPM cells). We demonstrated that cell proliferation was inhibited by phenytoin in a dose-dependent manner in both KD and HEPM cells. Co-treatment with SE restored phenytoin-induced toxicity in KD cells but did not protect HEPM cells against phenytoin-induced toxicity. Several microRNAs (miR-27b, miR-133b, miR-205, miR-497-5p, and miR-655-3p) is reported to associate with cell proliferation in KD cells. We measured the seven kinds of microRNAs (miR27b-3p, miR-27b-5p, miR-133b, miR-205-3p, miR-205-5p, miR-497-5p, and miR-655-3p) and found that SE suppressed miR-27b-5p induced by phenytoin in KD cells. Furthermore, co-treatment with SE enhanced the expression of miR-27b-5p downstream genes (PAX9, RARA, and SUMO1). These results suggest that SE protects phenytoin-induced cell proliferation inhibition by modulating miR-27b-5p.</p>
収録刊行物
-
- Biomedical Research
-
Biomedical Research 44 (2), 73-80, 2023-04-02
バイオメディカルリサーチプレス