<p>Background: The need for training for Yogo teachers is highest in first aid-related areas, regardless of the number of years of experience. In particular, head injuries may threaten the lives of children, thus requiring enhanced first-aid abilities, ranging from preventive measures to responsive actions. In this study, training was implemented using a basic knowledge test incorporating the minimum required knowledge for enhancing the first-aid abilities of Yogo teachers in the event of a head injury at school, and the acquisition of knowledge was measured. The study was aimed at obtaining suggestions concerning the effectiveness of the training and points to be added, based on the results of the basic knowledge test incorporating the seminar content.</p><p>Methods: This training was implemented as part of training held by the boards of education of three districts. Among the 201 participating Yogo teachers, 152 in Group A received classroom lectures only, while 49 in Group B experienced lectures, hands-on exercises, etc. Comparison was made between Group A and B, and based on the years of experience. A basic knowledge test comprising 30 questions was administered to all participants before and after the training.</p><p>Outcome: The rate of correct answers rose significantly both in Group A and in Group B after training. There was no difference in the rate of correct answers based on the years of experience, in Group A or among Yogo teachers with experience of less than ten years. As a point to be strengthened, necessity was suggested for more detailed explanation concerning actions in cases where the student has impaired consciousness, or where no problems are found in conversation with the student.</p><p>Conclusion: In training for enhancing first-aid abilities of Yogo teachers in the event of a head injury at school, the top priority should be placed on the acquisition of the minimum required knowledge that Yogo teachers must have. In this study, no difference was found based on years of experience before and after the training, in the level of knowledge acquisition (correct answer rate) concerning head injuries. It was suggested that nearly 90％ of participants could acquire the required level of knowledge by simply taking half-day classes without physical assessment or group work, if the classes are prepared with specified content based on the basic knowledge test.</p>