Nattokinase (Bac s 1), a subtilisin family serine protease, is a novel allergen contained in the traditional Japanese fermented food natto

DOI Web Site Web Site 被引用文献1件 参考文献30件 オープンアクセス
  • Suzuki Kayoko
    Department of Allergology, Fujita Health University School of Medicine
  • Nakamura Masashi
    Department of Integrative Medical Science for Allergic Disease, Fujita Health University School of Medicine General Research and Development Institute, Hoyu Co., Ltd.
  • Sato Nayu
    Department of Integrative Medical Science for Allergic Disease, Fujita Health University School of Medicine General Research and Development Institute, Hoyu Co., Ltd.
  • Futamura Kyoko
    Department of Allergology, Fujita Health University School of Medicine
  • Matsunaga Kayoko
    Department of Allergology, Fujita Health University School of Medicine Department of Integrative Medical Science for Allergic Disease, Fujita Health University School of Medicine
  • Yagami Akiko
    Department of Allergology, Fujita Health University School of Medicine

抄録

<p>Background: Immediate allergy caused by natto, a popular Japanese food prepared by fermenting soybeans with Bacillus subtilis var. natto, has been reported. Polygamma glutamic acid (PGA) in the sticky substance around natto beans has been reported to be a causative allergen of natto allergy. However, some of our patients with natto allergy were negative for PGA in the skin prick test (SPT). The sticky substance of natto beans contains a subtilisin family serine protease, nattokinase, along with PGA. In this study, we aimed to examine the antigenicity of nattokinase in natto allergy.</p><p>Methods: Eight patients, who developed symptoms after ingesting natto and positively reacted to natto (seven to the sticky substance in natto and one to the whole natto product) in their SPT, were enrolled in this study. To analyze IgE reactivity, we performed immunoblotting, ELISA, and SPT for natto (bean and sticky substance), and/or PGA, and/or nattokinase and/or cultured B. subtilis var. natto extract.</p><p>Results: In the SPT, four cases each were PGA-positive and PGA-negative. Immunoblotting of the sera from PGA-negative patients showed a protein band at 30 kDa, which was identified as nattokinase. Three PGA-negative cases, but not three PGA-positive cases, showed a positive reaction to nattokinase in the SPT and had a history of atopic dermatitis. The ELISA for nattokinase revealed a positive reaction of PGA-negative cases and negative reaction of PGA-positive cases in the SPT.</p><p>Conclusions: We identified a subtilisin family serine protease, nattokinase, as a novel allergen in natto allergy patients unsensitized to PGA.</p>

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (30)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ