Usefulness of SARS-CoV-2 antigen quantitative assay

MURATA Tatsuya, KOHARA Kengo, KASHIMA Hijiri, HIRAI Mirai, MARUYAMA Kyohei, IKEDA Mitsuyasu, FUKUOKA Tatsuhito, YUJIRI Toshiaki

doi:10.14932/jamt.22-61

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Other Title

SARS-CoV-2抗原定量検査の活用方法と有用性の検証
SARS-CoV-2 コウゲンテイリョウケンサノカツヨウホウホウトユウヨウセイノケンショウ

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Abstract

<p>The gold standard to detect SARS-CoV-2 is reverse transcription-polymerase chain reaction (RT-PCR). We evaluated an automated quantitative antigen assay using the Elecsys SARS-CoV-2 antigen (Roche) to detect SARS-CoV-2. We tested 214 subjects, 130 (60.7%) of whom were positive in the molecular tests for SARS-CoV-2 infection using nasopharyngeal samples, with a SARS-CoV-2 RT-PCR kit and the quantitative antigen assay. The positive concordance rate was 85.4% and the negative concordance rate was 100%. The Ct value of quantitative RT-PCR showed an inverse correlation with the logarithmically transformed antigen quantitative value (r = 0.947). Results suggest that the quantitative antigen assay is a potentially useful surrogate test for the quantitative RT-PCR assay since it also showed an acceptable accuracy as a screening test. The automated antigen assay also allows fast turn-around times and a high throughput and might be useful in emergency care, adequate bed management, and so forth.</p>

Journal

Japanese Journal of Medical Technology

Japanese Journal of Medical Technology 72 (2), 205-209, 2023-04-25

Japanese Association of Medical Technologists

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