Senescence-associated secretory phenotypes in rat-derived dedifferentiated fat cells with replicative senescence
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- DENG Wenqi
- Department of Orthodontics, Osaka Dental University
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- JO Jun-ichiro
- Department of Biomaterials, Osaka Dental University
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- MORIKUNI Hidetoshi
- Department of Orthodontics, Osaka Dental University
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- SASAYAMA Satoshi
- Department of Oral Implantology, Osaka Dental University
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- HASHIMOTO Yoshiya
- Department of Biomaterials, Osaka Dental University
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- MATSUMOTO Naoyuki
- Department of Orthodontics, Osaka Dental University
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- HONDA Yoshitomo
- Department of Oral Anatomy, Osaka Dental University
抄録
<p>Senescence-associated secretory phenotype (SASPs) secreted from senescent cells often cause the deleterious damages to the surrounding tissues. Although dedifferentiated fat (DFAT) cells prepared are considered a promising cell source for regenerative therapies, SASPs from DFAT cells undergoing long-term cell culture, which latently induce replicative senescence, have barely been explored. The present study was designed to investigate senescent behaviors in rat-derived DFAT cells at high passage numbers and to analyze the possible types of SASPs. Our data show that DFAT cells undergo senescence during replicative passaging, as determined by multiple senescent hallmarks including morphological changes in cell shape and nucleus. Moreover, RT2 PCR array analysis indicated that senescent DFAT cells expressed higher levels of 16 inflammatory cytokines (Ccl11, Ccl12, Ccl21, Ccl5, Csf2, Cxcl1, Cxcl12, Ifna2, IL11, IL12a, IL13, IL1a, IL1rn, IL6, Mif, and Tnf) associated with SASPs than non-senescent cells. This study implicates that rat DFAT cells undergo cellular senescence after long-term cell culture; cautious consideration should be paid to treat SASP secretion when senescent DFAT cells are used in regenerative medicine.</p>
収録刊行物
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- Dental Materials Journal
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Dental Materials Journal 42 (3), 351-359, 2023-05-25
一般社団法人 日本歯科理工学会