A simple method for species identification of the ghost crabs using PCR-RFLP
-
- Yuhara Takeshi
- Aquatic Ecology Laboratory, Graduate School of Life Sciences, Tohoku University
-
- Ohtsuki Hajime
- Aquatic Ecology Laboratory, Graduate School of Life Sciences, Tohoku University
-
- Urabe Jotaro
- Aquatic Ecology Laboratory, Graduate School of Life Sciences, Tohoku University
この論文をさがす
抄録
<p>Ghost crabs (genus Ocypode spp.) found on sandy beaches are susceptible with the loss of an ecotone, making them useful as indicators of anthropogenic alteration, such as seawall construction. On the sandy beaches of Sendai Bay on the Pacific coast of the Tohoku region of northern Japan, three Ocypode species have been sympatrically observed in recent years: O. stimpsoni, O. ceratophthalmus, and O. sinensis. However, these three Ocypode species only occur sympatrically from late summer to autumn, immediately after new recruitment. Since they are all juveniles at that period, it is difficult to identify them by morphological characteristics. Thus, we have developed a method for identifying these three Ocypode species. By using two restriction enzymes of AciI and BfaI, we can perform polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis on the 12S-rRNA region of the mitochondrial DNA. As a result, our PCR-RFLP analysis is able to identify all three Ocypode species. Therefore, this method will be a helpful tool for understanding population dynamics and evaluating the sandy beach ecosystem in cases where the three Ocypode species occur sympatrically but identification by morphological features is difficult.</p>
収録刊行物
-
- Plankton and Benthos Research
-
Plankton and Benthos Research 18 (2), 106-109, 2023-05-31
日本プランクトン学会、日本ベントス学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390577740952224000
-
- NII書誌ID
- AA12130745
-
- ISSN
- 1882627X
- 18808247
-
- NDL書誌ID
- 033116666
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
-
- 抄録ライセンスフラグ
- 使用不可