Species-specific Primer and Probe Sets for Detection of Syntrophic Long-chain Fatty Acid-degrading Bacteria in Anaerobic Digestion Using Quantitative PCR
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- Sakurai Riku
- Laboratory of Sustainable Animal Environment, Graduate School of Agricultural Science, Tohoku University
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- Fukuda Yasuhiro
- Laboratory of Sustainable Animal Environment, Graduate School of Agricultural Science, Tohoku University
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- Tada Chika
- Laboratory of Sustainable Animal Environment, Graduate School of Agricultural Science, Tohoku University
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説明
<p>Lipid-rich wastes are energy-dense substrates for anaerobic digestion. However, long-chain fatty acids (LCFAs), key intermediates in lipid degradation, inhibit methanogenic activity. In this study, TaqMan-based qPCR assays targeting the 16S rRNA gene of the cardinal LCFA-degrading bacterial species Syntrophomonas palmitatica and S. zehnderi were developed and validated. A trial experiment showed the advantage of species-specific quantification versus genus-specific quantification in assessing bacterial capacity for lipidic waste degradation. These qPCR assays will serve as monitoring tools for estimating the LCFA-degrading capacity of anaerobic digester communities and developing an effective strategy to enrich LCFA-degrading bacteria.</p>
収録刊行物
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- Microbes and environments
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Microbes and environments 38 (2), n/a-, 2023
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境生物学会
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詳細情報 詳細情報について
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- CRID
- 1390578050260124544
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- NII書誌ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 032941023
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- PubMed
- 37357389
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
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- 抄録ライセンスフラグ
- 使用不可