Relationship Between Rumen Microbial Composition and Fibrolytic Isozyme Activity During the Biodegradation of Rice Straw Powder Using Rumen Fluid

DOI Web Site Web Site 参考文献40件
  • Takizawa Shuhei
    Laboratory of Sustainable Animal Environment, Graduate School of Agricultural Science, Tohoku University Research Fellow of Japan Society for the Promotion of Science, Japan Society for the Promotion of Science
  • Asano Ryoki
    Department of Agro-Food Science, Faculty of Agro-Food Science, Niigata Agro-Food University
  • Abe Kenichi
    Department of Agro-Food Science, Faculty of Agro-Food Science, Niigata Agro-Food University
  • Fukuda Yasuhiro
    Laboratory of Sustainable Animal Environment, Graduate School of Agricultural Science, Tohoku University
  • Baba Yasunori
    Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
  • Sakurai Riku
    Laboratory of Sustainable Animal Environment, Graduate School of Agricultural Science, Tohoku University
  • Tada Chika
    Laboratory of Sustainable Animal Environment, Graduate School of Agricultural Science, Tohoku University
  • Nakai Yutaka
    Department of Agro-Food Science, Faculty of Agro-Food Science, Niigata Agro-Food University

この論文をさがす

説明

<p>Rumen fibrolytic microorganisms have been used to increase the rate of lignocellulosic biomass biodegradation; however, the microbial and isozymatic characteristics of biodegradation remain unclear. Therefore, the present study investigated the relationship between rumen microorganisms and fibrolytic isozymes associated with lignocellulosic biomass hydrolysis. Rice straw, a widely available agricultural byproduct, was ground and used as a substrate. The biodegradation of rice straw powder was performed anaerobically in rumen fluid for 48 h. The results obtained revealed that 31.6 and 23.3% of cellulose and hemicellulose, respectively, were degraded. The total concentration of volatile fatty acids showed a 1.8-fold increase (from 85.4 to 151.6‍ ‍mM) in 48 h, and 1,230.1‍ ‍mL L–1 of CO2 and 523.5‍ ‍mL L–1 of CH4 were produced. The major isozymes identified by zymograms during the first 12‍ ‍h were 51- and 140-kDa carboxymethyl cellulases (CMCases) and 23- and 57-kDa xylanases. The band densities of 37-, 53-, and 58-kDa CMCases and 38-, 44-, and 130-kDa xylanases increased from 24 to 36 h. A microbial ana­lysis indicated that the relative abundances of Prevotella, Fibrobacter, and Bacteroidales RF16 bacteria, Neocallimastix and Cyllamyces fungi, and Dasytricha and Polyplastron protozoa were related to fibrolytic isozyme activity. The present results provide novel insights into the relationships between fibrolytic isozymes and rumen microorganisms during lignocellulose biodegradation.</p>

収録刊行物

参考文献 (40)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ