Development and characterization of near-isogenic lines for brown planthopper resistance genes in the genetic background of <i>japonica</i> rice ‘Sagabiyori’

  • Shar Saw Bo Day
    The United Graduate School of Agricultural Sciences, Kagoshima University Loikaw Research Center, Department of Agricultural Research
  • Nguyen Cuong Dinh
    The United Graduate School of Agricultural Sciences, Kagoshima University Biotechnology Department, College of Food Industry
  • Sanada-Morimura Sachiyo
    Agro-Environment Research Division, Kyushu Okinawa Agricultural Research Center, NARO
  • Yasui Hideshi
    Plant Breeding Laboratory, Graduate School, Kyushu University
  • Zheng Shao-Hui
    Faculty of Agriculture, Saga University
  • Fujita Daisuke
    Faculty of Agriculture, Saga University

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  • Development and characterization of near-isogenic lines for brown planthopper resistance genes in the genetic background of japonica rice 'Sagabiyori'

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<p>The brown planthopper (BPH: Nilaparvata lugens Stål) is one of the most destructive insects in rice production. The use of host plant resistance has potential to reduce damage caused by BPH. The heat tolerance japonica rice ‘Sagabiyori’, with superior grain quality and high soluble starch in the stem, is highly susceptible to damage by BPH. Here, to enhance its BPH resistance, we developed seven near-isogenic lines (NILs) carrying BPH2, BPH17-ptb, BPH32, BPH3, BPH17, BPH20, and BPH21 through marker-assisted selection and evaluated resistance to two BPH populations. Most lines were more resistant to the Hadano-1966 BPH population than Sagabiyori but were less effective against the highly virulent Koshi-2013 population. Nevertheless, in antixenosis tests, Koshi-2013 settled less on all NILs than on Sagabiyori. In addition, adult mortality and the percentage of fresh weight loss of lines carrying BPH17 and BPH3 indicated that these lines have higher resistance to Koshi-2013 than Sagabiyori. Current study revealed that BPH resistance of Sagabiyori became stronger by transferring BPH3 and BPH17 genes. Thus, BPH3 and BPH17 might be valuable for breeding programs to enhance BPH resistance of high grain quality rice varieties with heat tolerance.</p>

Journal

  • Breeding Science

    Breeding Science 73 (4), 382-392, 2023

    Japanese Society of Breeding

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