植物トリテルペノイドの酵母生産: 実例および展望

DOI Web Site
  • 關 光
    大阪大学大学院工学研究科 大阪大学先導的学際研究機構
  • Soo Yeon Chung
    大阪大学大学院工学研究科
  • 村中 俊哉
    大阪大学大学院工学研究科 大阪大学先導的学際研究機構

書誌事項

タイトル別名
  • Advanced approaches used in heterologous production of plant-derived triterpenoids in engineered yeast
  • ショクブツ トリテルペノイド ノ コウボ セイサン : ジツレイ オヨビ テンボウ

この論文をさがす

抄録

<p>Triterpenoids and their glycosides, referred to as saponins, are a class of structurally diverse plant specialized metabolites. Although the biological role of triterpenoids in plants remains largely unclear, these compounds have been shown to possess various medicinal properties. For instance, they may be anti-inflammatory, anti-carcinogenic, and antiviral. Several compounds like glycyrrhizin in licorice and ginsenosides in ginseng form major components in traditional herbal medicines published in Japanese pharmacopoeia. Triterpenoids often accumulate at low levels in plants and their chemical synthesis can be challenging due to their complex structures. Recently, remarkable progress has been made in engineering the production of various plant triterpenoids by utilizing microorganisms, particularly Saccharomyces cerevisiae, as alternative hosts. This review describes representative examples of de novo production of plant-derived triterpenoids in S. cerevisiae and strategies to improve productivity of target compounds, including strategies to enhance metabolic flux, pathway enzyme engineering to improve enzyme activity, engineering of the cytochrome P450 monooxygenase (CYP) - NADPH cytochrome P450 reductase (CPR) interaction to enhance electron transfer efficiency in order to optimize the microenvironment of CYP, and expansion of endoplasmic reticulum (ER) to increase the abundance of ER-associated enzymes and consequential improvement in metabolic capacity. Additionally, perspectives on the use of pathway compartmentalization and metabolite transport strategies to improve productivity of target compound are also discussed.</p>

収録刊行物

  • 植物の生長調節

    植物の生長調節 57 (2), 77-83, 2022

    一般社団法人 植物化学調節学会

関連プロジェクト

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ