書誌事項
- タイトル別名
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- Orengedokuto inhibits TNF-α-induced HAVIC calcification obtained from calcific aortic valve stenosis patients
抄録
<p>Although calcific aortic valve stenosis (CAVS) is the most common heart valve disease in aging society, there is no effective medical treatment. Although orengedokuto (OGT), a herbal medicine, was used for treating inflammatory symptoms, the effect of OGT on aortic valve calcification (AVC) is unknown. So, we aimed to discover whether OGT inhibits AVC. Calcified AV were obtained from CAVS patients. Human aortic valve interstitial cells (HAVICs) were isolated from AV by collagenase treatment and cultured in α-MEM with 10% FBS. The medium containing TNF-α (30 ng/mL) was replenished every 3-4 days. We acknowledged that TNF-α strongly accelerated HAVICs calcification. We found that OGT (10-30 μg/mL) inhibited TNF-α-induced HAVIC calcification, significantly, which maintained cell viability. The elevation of alkaline phosphatase activity and gene expression of bone morphogenetic protein 2 induced by TNF-α was significantly inhibited by OGT (10 μg/mL). OGT also inhibited the acceleration of AVC in spontaneously hypertensive rat caused by aging and hypertension confirmed in vivo. These data showed that OGT inhibited AVC both in vitro and in vivo. The molecular mechanism of OGT inhibiting AVC needs to be further clarified for establishing medical therapy of CAVS.</p>
収録刊行物
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- 日本薬理学会年会要旨集
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日本薬理学会年会要旨集 97 (0), 1-B-P-068-, 2023
公益社団法人 日本薬理学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390580217715355904
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- ISSN
- 24354953
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
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- 抄録ライセンスフラグ
- 使用不可