頭部固定下で社会相互作用するマウスの表情変化

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  • Facial expression changes in mice during social interaction under head fixation

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<p>Autism spectrum disorder (ASD) is a developmental disorder characterized by abnormal social communication and repetitive behaviors. To study the neural circuit abnormalities underlying these manifestations using ASD model mice, it is necessary to establish an experimental paradigm that can quantitatively evaluate the changes in the subject’s emotions toward conspecifics when it interacts with them. To this end, we conducted facial videography and machine-vision-based image analysis to examine the facial expressions of mice interacting with conspecifics under head fixation. A transparent acrylic box with holes in the front, in which an inanimate object, a male conspecific, or a female conspecific was presented, was placed in front of a male C57BL/6 subject mouse head-fixed over a freely-rotating cylindrical treadmill. Images of the face of the subject mouse were recorded by a CCD camera under infrared illumination. Comparisons of each movie with prototypical faces obtained with each stimulus indicated that the facial expression changes elicited by each stimulus were unique. Overall, this paradigm can be combined with two-photon calcium imaging to reveal abnormalities in social and emotional brain circuits in ASD model mice and will also be helpful to evaluate the effects of drugs that aim to improve possible abnormal social emotions in mouse models of neurodevelopmental and neuropsychiatric disorders.</p>

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