Changes in the expression of mexB, mexY, and oprD in clinical Pseudomonas aeruginosa isolates
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- MATSUMOTO Yoshimi
- SANKEN (The Institute of Scientific and Industrial Research), Osaka University
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- YAMASAKI Seiji
- SANKEN (The Institute of Scientific and Industrial Research), Osaka University Graduate School of Pharmaceutical Sciences, Osaka University Institute for Advanced Co-Creation Studies, Osaka University
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- HAYAMA Kouhei
- SANKEN (The Institute of Scientific and Industrial Research), Osaka University
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- IINO Ryota
- Institute for Molecular Science, National Institutes of Natural Sciences Graduate Institute for Advanced Studies, The Graduate University for Advanced Studies (SOKENDAI)
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- NOJI Hiroyuki
- Graduate School of Engineering, The University of Tokyo
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- YAMAGUCHI Akihito
- SANKEN (The Institute of Scientific and Industrial Research), Osaka University
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- NISHINO Kunihiko
- SANKEN (The Institute of Scientific and Industrial Research), Osaka University Graduate School of Pharmaceutical Sciences, Osaka University Center for Infectious Disease Education and Research, Osaka University
書誌事項
- タイトル別名
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- Changes in the expression of <i>mexB</i>, <i>mexY</i>, and <i>oprD</i> in clinical <i>Pseudomonas aeruginosa</i> isolates
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抄録
<p>Changes in expression levels of drug efflux pump genes, mexB and mexY, and porin gene oprD in Pseudomonas aeruginosa were investigated in this study. Fifty-five multidrug-resistant P. aeruginosa (MDRP) strains were compared with 26 drug-sensitive strains and 21 strains resistant to a single antibiotic. The effect of the efflux inhibitor Phe-Arg-β-naphthylamide on drug susceptibility was determined, and gene expression was quantified using real-time quantitative real-time reverse transcription polymerase chain reaction. In addition, the levels of metallo-β-lactamase (MBL) and 6′-N-aminoglycoside acetyltransferase [AAC(6′)-Iae] were investigated. Efflux pump inhibitor treatment increased the sensitivity to ciprofloxacin, aztreonam, and imipenem in 71%, 73%, and 29% of MDRPs, respectively. MBL and AAC(6′)-Iae were detected in 38 (69%) and 34 (62%) MDRP strains, respectively. Meanwhile, 76% of MDRP strains exhibited more than 8-fold higher mexY expression than the reference strain PAO1. Furthermore, 69% of MDRP strains expressed oprD at levels less than 0.01-fold of those in PAO1. These findings indicated that efflux pump inhibitors in combination with ciprofloxacin or aztreonam might aid in treating MDRP infections.</p>
収録刊行物
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- Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences
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Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences 100 (1), 57-67, 2024-01-11
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詳細情報 詳細情報について
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- CRID
- 1390580239850665600
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- NII書誌ID
- AA00785485
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- ISSN
- 13492896
- 03862208
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- NDL書誌ID
- 033319197
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- PubMed
- 38199247
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
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- 抄録ライセンスフラグ
- 使用不可