Utility of Stereo Microscopy in the Evaluation of Organic-Inorganic Composite Material

DOI
  • UWAZUMI Suguru
    Graduate School of Dentistry, Department of Oral Implantology, Osaka Dental University, Osaka, Japan
  • NAKAGAWA Masato
    Department of Oral Anatomy, Osaka Dental University, Osaka, Japan
  • MORINAGA Kenzo
    Department of Oral Implantology, Osaka Dental University, Osaka, Japan
  • HASHIMOTO Yoshiya
    Department of Biomaterials, Osaka Dental University, Osaka, Japan
  • HONDA Yoshitomo
    Department of Oral Anatomy, Osaka Dental University, Osaka, Japan
  • BABA Shunsuke
    Department of Oral Implantology, Osaka Dental University, Osaka, Japan

Abstract

<p>Microscopy is a traditional method to estimate the morphology or microstructure of biomaterials. However, the use of stereomicroscopy with transmitted light for the analysis of organic-inorganic composite materials has not been widely explored. In this study, we aimed to verify the usefulness of stereomicroscopy with epi- and trans-illumination and field emission-scanning electron microscopy (FE-SEM) using the bone-substitute material, vacuum-heated epigallocatechin-conjugated gelatin sponges containing β-tricalcium phosphate granules, as model material. Compared with FE-SEM, in situ stereomicroscopy with trans-illumination facilitated the easy observation of the β-tricalcium phosphate granule distribution in sponges. Compared with epi-illumination, transmitted-illumination enabled easy detection of the fibrous structure of epigallocatechin-conjugated gelatin at the intergranular spaces and in the pores of β-tricalcium phosphate granules. Our findings suggest that the use of a stereomicroscope with trans-illumination may facilitate the scanning of the microstructure of organic-inorganic composite materials during biomaterial fabrication.</p>

Journal

Keywords

Details 詳細情報について

  • CRID
    1390580328310550016
  • DOI
    10.11344/nano.15.88
  • ISSN
    21854734
    18835198
  • Text Lang
    en
  • Data Source
    • JaLC
  • Abstract License Flag
    Disallowed

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