Induction of mesenchymal stem cell (MSC) differentiation from iPS cells using MSC medium

  • WU Yufan
    Department of Biomaterials, Osaka Dental University
  • IWASAKI Kengo
    Division of Creative and Integrated Medicine, Advanced Medical Research Center, Translational Research Institute for Medical Innovation (TRIMI), Osaka Dental University
  • HASHIMOTO Yoshiya
    Department of Biomaterials, Osaka Dental University

抄録

<p>Mesenchymal stem cells (MSCs) and induced pluripotent stem (iPS) cells have great potential as cell sources for tissue engineering and regenerative medicine. This study aimed to investigate whether iPS cells can be differentiated into MSCs using MSCGM, a commercially available MSC culture system. The cells were characterized by flow cytometry, immunostaining, and gene expression analyses. We also examined their potential to differentiate into osteoblasts and chondrocytes. Our results showed that iPS cells cultured in MSCGM (iPS-MSCGM) exhibited a fibroblast-like morphology and expressed CD73 and CD90 genes, as well as positive markers for CD73, CD90, and CD105. Moreover, iPS-MSCGM cells demonstrated the ability to differentiate into osteoblasts and chondrocytes in vitro. This study demonstrates a new and simple method for inducing the differentiation of iPS cells to MSCs using MSCGM.</p>

収録刊行物

  • Dental Materials Journal

    Dental Materials Journal 43 (1), 20-27, 2024-01-25

    一般社団法人 日本歯科理工学会

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