Quantification of Proteins That Are Included in the PM<sub>2.5</sub> of Kanagawa Prefecture

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  • 神奈川県におけるPM<sub>2.5</sub>に含まれるタンパク質の定量

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<p>To confirm the contribution of ingredients derived from living things, the protein included in the PM2.5 collected in the urban and mountain areas of Kanagawa Prefecture during different seasons was determined by the micro BCA protein assay method. As a result, a certain amount of protein was detected in samples from both the urban and mountain areas. In addition, the protein concentrations of the PM2.5 showed daily fluctuations, and similar daily fluctuations were observed between the urban and mountain areas in the spring and summer, but different in the autumn and winter. On the other hand, the protein concentrations in the PM2.5 in the urban area tended to be higher than those in the mountain area in the autumn and winter. When the contribution rates of the protein to the mass concentrations of the PM2.5 were calculated, they were around 20% on average. Furthermore, the correlation between the protein concentrations and the concentrations of the organic carbon (OC) component and the correlation between the protein concentrations and the concentrations of the water soluble organic carbon (WSOC) component in PM2.5 tended to always be high regardless of the sampling locations and seasons. When OC and WSOC analyses were conducted using bovine serum albumin, as the common protein, quantitative responses were observed. Therefore, it was concluded that a considerable amount of the OC and WSOC components detected in PM2.5 originated from the protein. The importance of conducting research on the premise that the PM2.5 contains biologically-originated components such as protein was suggested, for a future study.</p>

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