Development of a highly sensitive fluorescence assay for orthophthalaldehyde in endoscopic disinfectants

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  • 内視鏡消毒剤中オルトフタルアルデヒドの高感度蛍光定量法の開発

Abstract

Endoscopes are automatically rinsed with a disinfectant containing 0.55% ortho-phthalaldehyde (OPA) prior to use in a dedicated machine to prevent bacterial infection. Because the disinfectant is gradually diluted with repeated use, it is necessary to monitor the concentration of disinfectant in the cleaning machine as needed to ensure that it is within the effective concentration range. In this paper, we report the development of a fluorescence assay for OPA in disinfectants with excellent sensitivity, accuracy, and specificity. The developed method showed good quantification of OPA in disinfectants in the range of 0.20-0.55%, and also gave good quantification at concentrations of 0.01% in increments of 0.27-0.33%, which is near the lower limit of the cleaning power of it. This method is expected to be used because it is more accurate and sensitive than current OPA monitors, and anyone can easily perform highly reproducible assay by simply mixing reagents and disinfectant.

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