Investigation of <i>Pseudocercospora nepheloides</i> Contamination Sources in a Plant Nursery Facility using PCR.

DOI

Bibliographic Information

Other Title
  • トルコギキョウ斑点病の特異的プライマーを用いたPCR法による検出と育苗期における伝染源の解明

Abstract

We investigated the theory that contaminated seeds and materials in a plant nursery facility during seedling rearing can act as infection sources of Pseudocercospora nepheloides. A PCR method using specific primers established to detect the pathogen. PCR tests allowed the detection of the pathogen in the seeds harvested from diseased plant cultivars. The aforementioned diseased seeds as well as seeds artificially infected with P.nepheloides spores were resulting in the emergence of seedlings bearing the typicial disease symptoms. Adherence and potential germination of spores on the nursing materials in the facility was investigated. Results revealed that spores could germinate on some planting materials such as cell trays. Overall results obtained from the present study suggest that contaminated seeds and materials in plant nursery facilities can act as P.nepheloides infection sources.

Journal

Details 詳細情報について

  • CRID
    1390581179980583168
  • DOI
    10.60399/fafrc.9.0_8
  • ISSN
    2759257X
    21894876
  • Text Lang
    ja
  • Data Source
    • JaLC
  • Abstract License Flag
    Allowed

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