Investigation of <i>Pseudocercospora nepheloides</i> Contamination Sources in a Plant Nursery Facility using PCR.
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- Nariyama Hideki
- Fukuoka Agriculture and Forestry Research Center㸪
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- Kikuhara Kenji
- Fukuoka Agriculture and Forestry Research Center㸪
Bibliographic Information
- Other Title
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- トルコギキョウ斑点病の特異的プライマーを用いたPCR法による検出と育苗期における伝染源の解明
Abstract
We investigated the theory that contaminated seeds and materials in a plant nursery facility during seedling rearing can act as infection sources of Pseudocercospora nepheloides. A PCR method using specific primers established to detect the pathogen. PCR tests allowed the detection of the pathogen in the seeds harvested from diseased plant cultivars. The aforementioned diseased seeds as well as seeds artificially infected with P.nepheloides spores were resulting in the emergence of seedlings bearing the typicial disease symptoms. Adherence and potential germination of spores on the nursing materials in the facility was investigated. Results revealed that spores could germinate on some planting materials such as cell trays. Overall results obtained from the present study suggest that contaminated seeds and materials in plant nursery facilities can act as P.nepheloides infection sources.
Journal
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- Bulletin of The Fukuoka Agriculture and Forestry Research Center
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Bulletin of The Fukuoka Agriculture and Forestry Research Center 9 (0), 8-13, 2023
Fukuoka Agriculture and Forestry Research Center
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Details 詳細情報について
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- CRID
- 1390581179980583168
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- ISSN
- 2759257X
- 21894876
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- Text Lang
- ja
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- Data Source
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- JaLC
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- Abstract License Flag
- Allowed