Three-Dimensional Manipulation Technique Employing Optical Tweezers and Its Application: Mechanical Regulation via Nodal Cilia in Initiation of Left-right Symmetry Breaking

DOI

Bibliographic Information

Other Title
  • 光ピンセットによる3次元顕微操作技術と，体の左右軸決定における繊毛を介したメカニカルなシグナルの解明

Abstract

<p>Recent advancements in manipulation techniques, such as optical tweezers and magnetic tweezers, have facilitated precise manipulation of specimens under microscopic observation. This review introduces a three-dimensional (3-D) manipulation technique employing optical tweezers combined with the 3-D single-particle tracking method. Moreover, it highlights the cutting-edge research effectively utilizing these manipulation techniques.</p><p>The establishment of left-right (L-R) symmetry in the mammalian body relies on leftward fluid flow at the embryonic node, which activates nodal immotile cilia, referred to as the ‘antenna of the cell’, and activate left-side specific signal cascades. However, how the nodal flow activate the nodal immotile cilia were controversial. In our research, I and my colleagues utilized three-dimensional manipulation microscopy equipped with optical tweezers. Our findings revealed that nodal immotile cilia are responsive to mechanical stimuli, triggering calcium responses and Dand5 mRNA degradation. These events subsequently initiate left-side-specific signaling cascades responsible for the determination of left-right asymmetry.</p>

Journal

  • KENBIKYO

    KENBIKYO 59 (1), 37-40, 2024-04-30

    The Japanese Society of Microscopy

Keywords

Details 詳細情報について

  • CRID
    1390581622424861824
  • DOI
    10.11410/kenbikyo.59.1_37
  • ISSN
    24342386
    13490958
  • Text Lang
    ja
  • Data Source
    • JaLC
  • Abstract License Flag
    Disallowed

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