TALE-based C-to-T base editor for multiple homologous genes with flexible precision

DOI Web Site Web Site 参考文献28件 オープンアクセス
  • Hosoda Ayako
    Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Science, The University of Tokyo
  • Nakazato Issei
    Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Science, The University of Tokyo Research Fellow of Japan Society for the Promotion of Science
  • Okuno Miki
    Division of Microbiology, Department of Infectious Medicine, Kurume University School of Medicine
  • Itoh Takehiko
    School of Life Science and Technology, Tokyo Institute of Technology
  • Takanashi Hideki
    Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Science, The University of Tokyo
  • Tsutsumi Nobuhiro
    Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Science, The University of Tokyo
  • Arimura Shin-ichi
    Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Science, The University of Tokyo

書誌事項

公開日
2024-12-25
資源種別
journal article
DOI
  • 10.5511/plantbiotechnology.24.0510a
公開者
日本植物バイオテクノロジー学会

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説明

<p>Recently a cytidine deaminase-based method for highly efficient C-to-T targeted base editing was developed and has been used with CRISPR-mediated systems. It is a powerful method for genome engineering, although it is prone to off-target effects and has a limited targeting scope. Transcription activator-like effector (TALE)-based tools which allow longer recognition sequences than do CRISPR/Cas9 systems, can also be used for targeted C-to-T base editing. Here, we describe a method that efficiently achieved targeted C-to-T substitutions in Arabidopsis nuclear genes using cytidine deaminase fused to a TALE DNA-binding domain. We used a single pair of TALEs with a novel TALE-repeat unit that can recognize all four DNA bases, especially to allow for variations in the third base of codons in homologous genes. This targeting strategy makes it possible to simultaneously base edit almost identical sites in multiple isoforms of a gene while suppressing off-target substitutions.</p>

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