A Study to Improve the Success Rate of the RNA Analysis of the Oncomine™ Dx Target Test Multi-CDx System

Iketani Akemi, Sakaguchi Tadashi, Ito Kentaro, Yamada Kenta, Inagaki Hiromi, Katsuta Koji, Hataji Osamu

doi:10.2482/haigan.65.24

<p>Objective. The objective of this study was to evaluate the impact on test results of implementing measures to prevent contamination by nucleic acid degradation enzymes (nucleic acid degradation prevention measures) on the protocol established by our hospital during the specimen preparation process, in cases of invalid RNA analysis results using the Oncomine™ Dx Target Test multi-CDx system (ODxTT). Methods. This retrospective study included consecutive patients diagnosed with non-small cell lung cancer (NSCLC) at our hospital between April 2021 and March 2022, whose samples were submitted for testing using the ODxTT. The ODxTT and RNA analysis results were compared between 50 cases tested before the implementation of nucleic acid degradation prevention measures (pre-implementation group) and 173 cases tested after the implementation of these measures (post-implementation group). Results. The analysis success rate of ODxTT was 88.0% in the pre-implementation group and 98.8% in the post-implementation group; the improvement in the post-implementation group was statistically significant (p < 0.01). Invalid RNA results were obtained in 5 cases (10%) in the pre-implementation group and 0% in the post-implementation group. Conclusion. Prevention of nucleolytic enzyme contamination during sample preparation process for ODxTT may improve the success rate of RNA analysis.</p>

A Study to Improve the Success Rate of the RNA Analysis of the Oncomine™ Dx Target Test Multi-CDx System

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