Principles of electrophoresis and comparison of buffer systems : from SDS-PAGE to Native-PAGE

DOI IR (HANDLE) Open Access

Bibliographic Information

Other Title
  • 電気泳動の原理とbuffer系の比較 : SDS-PAGEからNative-PAGEへ

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Description

Gel electrophoresis is a widely used technique for the separation of proteins and protein complexes. This paper discusses three critical considerations in gel electrophoresis: 1) selection of the chemical that gives negative charge to the protein, 2) selection of the gel matrix, and 3) selection of the detergent. In addition, I review the historical　development and underlying principles of buffer systems used in SDS-PAGE, blue-native (BN) PAGE, and clearnative (CN) PAGE. Special attention is given to the standard buffer systems used in SDS-PAGE and CN-PAGE, as well as modified buffer systems, two of which have been developed by the author.

Journal

  • 低温科学

    低温科学 83 221-228, 2025-03-29

    低温科学第83巻編集委員会および光合成ユビキティ共同編集

Keywords

Details 詳細情報について

  • CRID
    1390585803078108928
  • DOI
    10.14943/lowtemsci.83.221
  • HANDLE
    2115/94802
  • ISSN
    18807593
  • Text Lang
    ja
  • Article Type
    departmental bulletin paper
  • Data Source
    • JaLC
    • IRDB
  • Abstract License Flag
    Allowed

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