Evaluation of a new screening agar, Pourmedia<sup>®</sup> Vi Haemophilus agar, for <i>Haemophilus influenzae</i>

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  • 新規<i>Haemophilus influenzae</i>スクリーニング培地の検討―ポアメディア<sup>®</sup>Viヘモフィルス寒天培地の有用性評価―

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<p>Haemophilus influenzae causes respiratory tract infections and other invasive infections, and requires appreciable technical skill for isolation from chocolate agar (CHO). In this study, we evaluated the performance of Pourmedia® Vi Haemophilus agar (Vi), a new screening agar for H. influenzae. Clinical respiratory specimens, such as sputum and pharyngeal mucus, were inoculated onto either CHO (conventional method) or chocolate agar with bacitracin (Baci) and Vi (Vi method), followed by incubation at 35°C for 18 to 24 h in a 5% CO2-enriched atmosphere. After incubation, colonies suspected to be H. influenzae were isolated from each agar plate and identified using MALDI Biotyper. Counts of H. influenzae colonies detected and agreements between the two methods were compared, i.e., comparison between the usual test to detect H. influenzae (conventional method) and the new test using Vi (Vi method). From the 272 samples, 53 strains of Haemophilus spp. and Haemophilus-like organisms were isolated by the conventional method, compared with 55 strains isolated by the Vi method. Twenty-six strains (49.1%) and 31 strains (56.4%) of H. influenzae were confirmed by the conventional method and the Vi method, respectively. In this study, the Vi method was found to be more efficient than the conventional method, both in terms of the count of H. influenzae detected and agreement between the two methods. Furthermore, because the Vi method permits the examination of hemolytic properties after primary culture, the verification test is simplified, leading to enhanced utility for the isolation of H. influenzae from clinical specimens.</p>

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