Genetic and tissue-specific RNA-sequencing analysis of self-compatible mutant TSC28 in Brassica rapa L. toward identification of a novel self-incompatibility factor
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- Osaka Masaaki
- Graduate School of Life Sciences, Tohoku University
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- Nabemoto Moe
- Graduate School of Life Sciences, Tohoku University
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- Maeda Shunsuke
- Graduate School of Life Sciences, Tohoku University
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- Sakazono Satomi
- Graduate School of Life Sciences, Tohoku University
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- Masuko-Suzuki Hiromi
- Graduate School of Life Sciences, Tohoku University
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- Ito Kana
- Graduate School of Life Sciences, Tohoku University
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- Takada Yoshinobu
- Graduate School of Life Sciences, Tohoku University
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- Kobayashi Issei
- Advanced Science Research Promotion Center, Mie University
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- Lim Yong Pyo
- Department of Horticulture, Chungnam National University
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- Nakazono Mikio
- Graduate School of Bioagricultural Sciences, Nagoya University
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- Fujii Sota
- Graduate School of Agriculture and Life Sciences, The University of Tokyo
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- Murase Kohji
- Graduate School of Agriculture and Life Sciences, The University of Tokyo
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- Takayama Seiji
- Graduate School of Agriculture and Life Sciences, The University of Tokyo
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- Suzuki Go
- Division of Natural Science, Osaka Kyoiku University
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- Suwabe Keita
- Graduate School of Bioresources, Mie University
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- Watanabe Masao
- Graduate School of Life Sciences, Tohoku University
書誌事項
- タイトル別名
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- Genetic and tissue-specific RNA-sequencing analysis of self-compatible mutant TSC28 in <i>Brassica rapa</i> L. toward identification of a novel self-incompatibility factor
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<p>Self-incompatibility (SI) is a sophisticated system for pollen selectivity to prevent pollination by genetically identical pollen. In Brassica, it is genetically controlled by a single, highly polymorphic S-locus, and the male and female S-determinant factors have been identified as S-locus protein 11 (SP11)/S-locus cysteine-rich protein (SCR) and S-locus receptor kinase (SRK), respectively. However, the overall molecular system and identity of factors in the downstream cascade of the SI reaction remain unclear. Previously, we identified a self-compatible B. rapa mutant line, TSC28, which has a disruption in an unidentified novel factor of the SI signaling cascade. Here, in a genetic analysis of TSC28, using an F2 population from a cross with the reference B. rapa SI line Chiifu-401, the causal gene was mapped to a genetic region of DNA containing markers BrSA64 and ACMP297 in B. rapa chromosome A1. By fine mapping using an F2 population of 1,034 plants, it was narrowed down to a genetic region between DNA markers ACMP297 and BrgMS4028, with physical length approximately 1.01 Mbp. In this genomic region, 113 genes are known to be located and, among these, we identified 55 genes that were expressed in the papilla cells. These are candidates for the gene responsible for the disruption of SI in TSC28. This list of candidate genes will contribute to the discovery of a novel downstream factor in the SP11–SRK signaling cascade in the Brassica SI system.</p>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 94 (4), 167-176, 2019-08-01
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390845702307219072
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- NII論文ID
- 130007738050
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 030112571
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- PubMed
- 31474624
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 使用不可