A Simple Spectrophotometric Assay for Determination of Selenium Concentration in the Hospital Preparation

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  • Ito Miyabi
    Department of Pharmacy, University of Tsukuba Hospital
  • Shimada Saori
    Department of Pharmacy, University of Tsukuba Hospital
  • Yokota Naoyuki
    Department of Pharmacy, University of Tsukuba Hospital
  • Doki Kosuke
    Department of Pharmacy, University of Tsukuba Hospital Department of Pharmaceutical Sciences, Faculty of Medicine, University of Tsukuba
  • Homma Masato
    Department of Pharmacy, University of Tsukuba Hospital Department of Pharmaceutical Sciences, Faculty of Medicine, University of Tsukuba

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Other Title
  • 吸光光度法による院内製剤セレン注射液の簡易定量試験法

Abstract

<p>A simple spectrophotometric assay was developed for quantitative determination of selenium in selenium injection which is a hospital preparation for patients with selenium deficiency. Aliquots of the samples (500 µL) added 20 µL of 2.5 M formic acid and 20 µL of 18.5 mM O-phenylene diamine were incubated for two hours at room temperature. The absorbance was measured in the mixture at 333 nm by using NanoDrop®, a convenient instrument for spectrophotometric assay. The standard curve for determining selenium concentration was linear in the range of 1.25-30 µg/mL (r > 0.999). Coefficients of variation for intra- and inter-day assays were 0.3 to 5.5％ and 0.8 to 2.8％, respectively. Assay validation was confirmed by five independent measurements of 50 µg/mL selenium injection in three pharmacists with the accuracy of 0.0 to 1.6％. This assay method was successfully applied to the quantitative determination of selenium in selenium injection.</p>

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