Characterization of rice KT/HAK/KUP potassium transporters and K⁺ uptake by HAK1 from Oryza sativa
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- Okada Tomoyuki
- Faculty of Agriculture, Kochi University Graduate School of Biological Sciences, Nara Institute of Science and Technology Kochi Agricultural Research Center
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- Yamane Sousuke
- Faculty of Agriculture, Kochi University
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- Yamaguchi Masatoshi
- Graduate School of Biological Engineering, Saitama University
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- Kato Ko
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Shinmyo Atsuhiko
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Tsunemitsu Yuta
- Faculty of Agriculture, Kochi University
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- Iwasaki Kozo
- Faculty of Agriculture, Kochi University
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- Ueno Daisei
- Faculty of Agriculture, Kochi University
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- Demura Taku
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
書誌事項
- タイトル別名
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- Characterization of rice KT/HAK/KUP potassium transporters and K<sup>+</sup> uptake by HAK1 from <i>Oryza sativa</i>
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<p>Plant high-affinity K+ (HAK) transporters are divided into four major clusters. Cluster I transporters, in particular, are thought to have high-affinity for K+. Of the 27 HAK genes in rice, eight HAK transporters belong to cluster I. In this study, we investigated the temporal expression patterns during K+ deficiency and K+ transport activity of these eight HAK transporters. The expression of seven HAK genes except OsHAK20 was detected. Expression of OsHAK1, OsHAK5 and OsHAK21 was induced in response to K+ deficiency; however, that of other genes was not. Six of the eight HAK transporters—OsHAK1, OsHAK5, OsHAK19, OsHAK20, OsHAK21, and OsHAK27—complemented the K+-transporter-deficient yeast or bacterial strain. Further, the yeast cells expressing OsHAK1 were more sensitive to Na+ than those expressing OsHAK5. Mutant analysis showed that the high-affinity K+ uptake activity was almost undetectable in oshak1 mutants in a low-K+ medium (0.02 mM). In addition, the high-affinity K+ uptake activity of wild-type plants was inhibited by mild salt stress (20 mM NaCl); however, Na+ permeability of OsHAK1 was not detected in Escherichia coli cells. The high-affinity K+ uptake activity by leaf blades was detected in wild-type plants, while it was not detected in oshak1 mutants. Our results suggest that OsHAK1 and OsHAK5 are the two important components of cluster I corresponding to low-K+ conditions, and that the transport activity of OsHAK1, unlike that of OsHAK5, is sensitive to Na+. Further, OsHAK1 is suggested to involve in foliar K+ uptake.</p>
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 35 (2), 101-111, 2018-06-25
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390845712973049984
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- NII論文ID
- 130007393718
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 029091731
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可