PCR-RFLP Method for Rapid Discrimination of Toxic Plants Involved in Food Poisoning

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  • 食中毒事例の多い有毒植物のPCR-RFLP法による鑑別
  • ショクチュウドク ジレイ ノ オオイ ユウドク ショクブツ ノ PCR-RFLPホウ ニ ヨル カンベツ

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Abstract

<p>Food poisoning caused by natural toxins, especially poisonous plants, is characterized by severe symptoms and a relatively high mortality rate. Therefore, rapid and accurate identification of the causative agent is extremely important. From plant toxin food poisoning data published by the Ministry of Health, Labour and Welfare of Japan from 1989 to 2015, we selected five plants (Veratrum spp., Datura spp., Aconitum spp., Narcissus spp. and Colchicum spp.) that are frequently involved in poisoning outbreaks, and developed a PCR-RFLP assay to discriminate them. Separation of the PCR-RFLP products by electrophoresis resulted in detection of two fragments from poisonous plants and one from edible plants. The PCR-RFLP method is rapid and straightforward and does not require expensive analytical devices. This assay was also confirmed to be applicable to cooked samples.</p>

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