Cloning and Heterologous Expression of the Agrobactin Biosynthetic Gene Cluster from a Marine Metagenome
-
- Fujita Masaki
- 北大創成研
-
- Tokita Takayuki
- 北大院水産
-
- Ishikawa Takafumi
- 北大院水産
-
- Sakai Ryuichi
- 北大院水産
Bibliographic Information
- Other Title
-
- 海綿メタゲノムからのアグロバクチン生合成遺伝子クラスターの取得と異宿主発現
Abstract
<p>A biosynthetic gene cluster of unknown siderophore was cloned from a metagenomic library constructed from a marine sponge by function based screening method. Sequencing of the cloned DNA revealed presence of a putative biosynthetic gene cluster consisted of 15 ORFs which showed similarity to vibriobactin biosynthetic gene cluster from Vibrio cholera. </p><p> Culture broth of the siderophore producing clone exhibited characteristic mass peak at m/z 637 which rack in the negative clone. The crude extract of the broth was fractionated by repetitive column chromatography guided by mass signal. The product was finally purified by HPLC as hexamethyl derivative. Structure of the methylated product was determined by combination of spectroscopic analysis as well as speculation from biosynthetic information to be a hexamethyl agrobactin suggested that the original product was agrobaction which has been reported from a terrestrial plant pathogen Agrobacterium tumefaciens.</p><p> It was the first report of agrobaction from marine environmental samples and also the first heterologous production of this class of molecules.</p>
Journal
-
- Symposium on the Chemistry of Natural Products, symposium papers
-
Symposium on the Chemistry of Natural Products, symposium papers 56 (0), Poster55-, 2014
Symposium on the Chemistry of Natural Products Steering Committee
- Tweet
Details 詳細情報について
-
- CRID
- 1390845712975521152
-
- NII Article ID
- 130007399553
-
- ISSN
- 24331856
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- CiNii Articles
-
- Abstract License Flag
- Disallowed