Cloning and Heterologous Expression of the Agrobactin Biosynthetic Gene Cluster from a Marine Metagenome

DOI

Bibliographic Information

Other Title
  • 海綿メタゲノムからのアグロバクチン生合成遺伝子クラスターの取得と異宿主発現

Abstract

<p>A biosynthetic gene cluster of unknown siderophore was cloned from a metagenomic library constructed from a marine sponge by function based screening method. Sequencing of the cloned DNA revealed presence of a putative biosynthetic gene cluster consisted of 15 ORFs which showed similarity to vibriobactin biosynthetic gene cluster from Vibrio cholera. </p><p> Culture broth of the siderophore producing clone exhibited characteristic mass peak at m/z 637 which rack in the negative clone. The crude extract of the broth was fractionated by repetitive column chromatography guided by mass signal. The product was finally purified by HPLC as hexamethyl derivative. Structure of the methylated product was determined by combination of spectroscopic analysis as well as speculation from biosynthetic information to be a hexamethyl agrobactin suggested that the original product was agrobaction which has been reported from a terrestrial plant pathogen Agrobacterium tumefaciens.</p><p> It was the first report of agrobaction from marine environmental samples and also the first heterologous production of this class of molecules.</p>

Journal

Details 詳細情報について

  • CRID
    1390845712975521152
  • NII Article ID
    130007399553
  • DOI
    10.24496/tennenyuki.56.0_poster55
  • ISSN
    24331856
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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