新規蛍光プローブ探索のためのC2C12細胞を用いた機能性組織の再構築

  • 友 亮人
    前橋工科大学 システム生体工学科
  • 神 隆
    理化学研究所生命システム研究センター
  • 野村 保友
    前橋工科大学 システム生体工学科 理化学研究所生命システム研究センター

書誌事項

タイトル別名
  • Functional reorganization of excitable cell culture system for screening novel fluorescent probes in NIR-II
  • シンキ ケイコウ プローブ タンサク ノ タメ ノ C2C12 サイボウ オ モチイタ キノウセイ ソシキ ノ サイコウチク

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抄録

The second near-infrared window ranged between 900 and 1400 nm, namely NIR-II, has advantages for deep tissue imaging with extrinsic fluorophores due to lacking autofluorescence, low light absorption, and reduced scattering. Most of the proposed probes were used for labeling with antibodies. As a new application, we plan to monitor electrical activity in a living tissue noninvasively using membrane potential dye with NIR-II fluorescence. So we need the excitable cell culture system to screen candidates of such dyes. C2C12 skeletal myoblasts were morphologically differentiated to myotubes under the optimal conditions, and the electrical stimulation-induced contraction of the myotubes was monitored optically. This myotube system would permit screening membrane potential dyes in NIR-II.

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