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- 尾形 智夫
- 前橋工科大学 生物工学科
書誌事項
- タイトル別名
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- Construction of a brewing yeast expressing the glucoamylase gene STA1 by mating
- セツゴウ ニ ヨリ グルコアミラーゼ イデンシ STA1 ガ ハツゲン シタ ビール コウボ ノ イクシュ
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抄録
Standard brewing yeast cannot utilize larger oligomers or dextrins, which represent about 25% of wort sugars. A brewing yeast strain that could ferment these additional sugars to ethanol would be useful for producing low-carbohydrate diabetic or low-calorie beers. In this study, a brewing yeast strain that secretes glucoamylase was constructed by mating. The resulting Saccharomyces cerevisiae 278/113371 yeast was MATa/ diploid, but expressed the glucoamylase gene STA1. At the early phase of the fermentation test in malt extract medium, the fermentation rate of the diploid STA1 strain was slower than those of both the parent strain S. cerevisiae MAFF113371 and the reference strain bottom-fermenting yeast Weihenstephan 34/70. At the later phase of the fermentation test, however, the fermentation rate of the STA1 yeast strain was faster than those of the other strains. The concentration of ethanol in the culture supernatant of the STA1 yeast strain after the fermentation test was higher than those of the others. The concentration of all maltooligosaccharides in the culture supernatant of the STA1 yeast strain after the fermentation test was lower than those of the parent and reference strains, whereas the concentrations of flavor compounds in the culture supernatant were higher. These effects are due to the glucoamylase secreted by the constructed STA1 yeast strain. In summary, a glucoamylase-secreting diploid yeast has been constructed by mating that will be useful for producing novel types of beer owing to its different fermentation pattern and concentrations of ethanol and flavor compounds.
収録刊行物
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- 前橋工科大学研究紀要
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前橋工科大学研究紀要 21 (0), 53-56, 2018
公立大学法人 前橋工科大学
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詳細情報 詳細情報について
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- CRID
- 1390845712986890880
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- NII論文ID
- 130007435891
- 120007170612
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- NII書誌ID
- AA11225201
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- ISSN
- 24335673
- 13438867
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- NDL書誌ID
- 029169631
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可