DNA analysis of insects using reflex bleeding

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  • KATOH Toru
    Department of Natural History Sciences, Faculty of Science, Hokkaido University
  • FURUKAWA Kota
    Department of Natural Sciences, Graduate School of Science, Hokkaido University
  • KATAKURA Haruo
    Department of Natural History Sciences, Faculty of Science, Hokkaido University

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  • 反射出血を利用した昆虫類のDNA解析
  • ハンシャ シュッケツ オ リヨウシタ コンチュウルイ ノ DNA カイセキ

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Abstract

Reflex bleeding is characteristic for a number of insect species such as ladybeetles and leaf beetles. In this study, we assessed the utility of reflex bleeding as a noninvasive source for DNA analysis. By using the method based on the nucleotide-acid binding property of silica particles, we were able to extract DNA from the reflex bleeding exuded by some ladybeetles. In addition, using the extracted DNA, we were able to amplify and determine the nucleotide sequences of 28S region. These results suggest that the reflex bleeding will be a useful source for analyzing DNA in insects without killing them.

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