Influence of Platelet-rich Plasma on Human Dermal Papilla Cells and Its Application for Hair Development

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  • Miyano Ryotaro
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine
  • Sumie Rena
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine
  • Sugaya Humito
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine
  • Hayashi Kyoko
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine
  • Takeuchi Takayuki
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine
  • Fukuoka Yurie
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine
  • Wakisaka Nagaoki
    Wakisaka Osaka Clinic
  • Inoue Hajime
    Department of Plastic and Reconstructive Surgery, Division of Stem Cell Medicine (Endowed Chair by ANGFA Co.), St. Marianna University School of Medicine
  • Kajikawa Akiyoshi
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine

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Other Title
  • 多血小板血漿（PRP）のヒト培養毛乳頭細胞に及ぼす影響と育毛への効果

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<p>Background: To investigate the effects of platelet-rich plasma (PRP) on hair growth, the authors used molecular biology to investigate the influence of PRP on cultured human dermal papilla cells (DPC).<br/>Methods: PRP-treated DPC were cultured for a week. Gene expression levels of FGF-2, VEGF, BMP-2, Wnt5a, and EFNA3 were measured using PCR.<br/>Results: PRP significantly increased FGF-2, VEGF, and BMP-2 gene expression levels in DPC after 2 hours compared to those without PRP. After 24 hours, increased expression of Wnt5a was maintained. However, there was no significant difference in VEGF expression. BMP-2 expression was significantly decreased, but that of EFNA3 did not change. There were no changes in the expression levels of these genes in PRP with exosomes removed.<br/>Discussion: These results suggested that increased expression of hair growth-related genes in DPC activates hair follicles in the catagen phase of the hair cycle. Sustained expression of Wnt5a, and the short-term strong expression of BMP2 followed by its significant decrease suppress differentiation and cause atrophy of hair papillae.<br/>Conclusions: PRP is not involved in hair growth but is involved in hair development.</p>

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