The Effect of Cold Incubation before Mass Spectrometry Identification on Different <i>Saccharomyces cerevisiae </i>Strains

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  • 質量分析による<i>Saccharomyces cerevisiae</i>菌株識別に対する低温処理の影響
  • 質量分析によるSaccharomyces cerevisiae菌株識別に対する低温処理の影響
  • シツリョウ ブンセキ ニ ヨル Saccharomyces cerevisiae キンカブ シキベツ ニ タイスル テイオン ショリ ノ エイキョウ

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Abstract

<p>This study was carried out to evaluate the effect of cold stress on matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) cluster analysis of Saccharomyces cerevisiae. Seven pair strains including one bread yeast strain and one or two wild type strains of S. cerevisiae were selected from yeast strain stocks at the Aichi Center for Industry and Science Technology. By using MALDI-TOF MS cluster analysis, those pair strains were shown as being not separated into single strain clusters. When those colonies were re-incubated at low temperatures before MALDI-TOF MS preparation, all pair strains were found to have separated into single strain clusters. These distinctions show that cold stress proteins could be used as biomarkers for MALDI-TOF MS identification of S. cerevisiae strains.</p>

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