Evidence for the involvement of FXR signaling in ovarian granulosa cell function
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- TAKAE Kentaro
- Laboratory of Applied Reproductive Science, Faculty of Agriculture, Shinshu University, Nagano 399-4598, Japan
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- NAKATA Mizuho
- Laboratory of Applied Reproductive Science, Faculty of Agriculture, Shinshu University, Nagano 399-4598, Japan
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- WATANABE Takafumi
- Laboratory of Animal Functional Anatomy, Faculty of Agriculture, Shinshu University, Nagano 399-4598, Japan
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- SASADA Hiroshi
- Laboratory of Animal Reproduction, School of Veterinary Medicine, Kitasato University, Aomori 034-8628, Japan
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- FUJII Hiroshi
- Laboratory of Biochemistry, Faculty of Agriculture, Shinshu University, Nagano 399-4598, Japan Department of Interdisciplinary Genome Sciences and Cell Metabolism, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Nagano 399-4598, Japan
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- TOMIOKA Ikuo
- Laboratory of Applied Reproductive Science, Faculty of Agriculture, Shinshu University, Nagano 399-4598, Japan Department of Interdisciplinary Genome Sciences and Cell Metabolism, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Nagano 399-4598, Japan
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<p> Farnesoid X receptor (FXR) is mainly present in enterohepatic tissues and regulates cholesterol, lipid, and glucose homeostasis in coordination with target genes such as SHP and FABP6. Although FXR has been revealed to be expressed in reproductive tissues, FXR function and expression levels in the ovary remain unknown. In this study, we investigated FXR expression in mouse ovaries and its target genes in ovarian granulosa cells. In situ hybridization and immunohistochemical staining showed that FXR was mainly distributed in secondary and tertiary follicles. The agonist-induced activation of FXR in cultured granulosa cells induced the expression of SHP and FABP6, while siRNA targeting of FXR decreased CYP19a1 and HSD17b1 expression. Upon examination of the roles of SHP and FABP6 in granulosa cells, we found that SHP overexpression significantly decreased StAR, CYP11a1, and HSD3b gene expression. In addition, siRNA targeting of FABP6 decreased CYP19a1 and HSD17b1 expression, while FABP6 overexpression increased CYP19a1 expression. In conclusion, the present study demonstrates the presence of FXR signaling in the ovary and reveals that FXR signaling may have a role in function of granulosa cells.</p>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 65 (1), 47-55, 2019
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390845713052305792
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- NII論文ID
- 130007593830
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- HANDLE
- 10659/00006631
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- NDL書誌ID
- 029510923
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- PubMed
- 30449821
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 使用不可