Development of microsatellite markers for the completely cleistogamous species <i>Gastrodia takeshimensis</i> (Orchidaceae) that are transferable to its chasmogamous sister <i>G. nipponica</i>

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  • Kishikawa Keiju
    Graduate School of Symbiotic Systems Science and Technology, Fukushima University
  • Suetsugu Kenji
    Department of Biology, Graduate School of Science, Kobe University
  • Kyogoku Daisuke
    Present affiliation: Graduate School of Life Sciences, Tohoku University Graduate School of Agriculture, Kyoto University
  • Ogaki Kenji
    Graduate School of Symbiotic Systems Science and Technology, Fukushima University
  • Iga Daisuke
    Faculty of Symbiotic Systems Science, Fukushima University
  • Shutoh Kohtaroh
    Graduate School of Symbiotic Systems Science and Technology, Fukushima University Faculty of Education, Niigata University
  • Isagi Yuji
    Graduate School of Agriculture, Kyoto University
  • Kaneko Shingo
    Faculty of Symbiotic Systems Science, Fukushima University

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  • Development of microsatellite markers for the completely cleistogamous species Gastrodia takeshimensis (Orchidaceae) that are transferable to its chasmogamous sister G. nipponica

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<p>We developed microsatellite markers to compare the genetic variation between the putatively cleistogamous Gastrodia takeshimensis (Orchidaceae) and its chasmogamous sister species G. nipponica. We expected low genetic variation in G. takeshimensis in view of its hypothesized cleistogamy. Eighteen primer pairs were developed from a G. takeshimensis genomic DNA library, and their characteristics were tested for G. takeshimensis and G. nipponica. Seven loci were polymorphic in G. nipponica, whereas all loci showed no polymorphism in G. takeshimensis. Genetic diversity was thus not detected in G. takeshimensis, and it seems to have been lost by repeated selfing in the completely closed flower. The 18 markers described here will be useful for investigating the genetic variation between a cleistogamous species and its chasmogamous sister species.</p>

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