Effect of titanium substrate photofunctionalization by UV irradiation on proliferation and differentiation of osteoblastic cell

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  • 紫外線照射によるチタン基板の光機能化が骨芽細胞の増殖・分化に与える影響

Abstract

<p>This paper presents the influence of surface condition of titanium on cell adhesion, cell activity and cell differentiation. It is well known that photofunctionalization on titanium surfaces promotes the osteogenesis. It is common knowledge that cells strongly adhere to UV irradiated titanium substrate because UV surface modification improves affinity of titanium surface and cells. However, there are not many studies investigating the influence on cell dynamics and cellular activity changes by VUV irradiation time. In this study, a titanium substrate treated with various VUV irradiation times was used. First, colorimetric analysis using methylene blue was carried out in order to investigate the properties of ultraviolet irradiation surface. Further osteoblasts were used. Cell morphology was evaluated by fluorescence microscopy. Cell activity and cell differentiation were evaluated using absorbance measurements. As a result of the experiment, Cell adhesion was improved by VUV irradiation. On the other hand, cell activity and ALP production amount changed depending on the irradiation time.</p>

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