Characterization of Rab phosphorylation by LRRK2 using Phos-tag SDS–PAGE

DOI Web Site 12 References
  • Ito Genta
    Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
  • Tomita Taisuke
    Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan

Bibliographic Information

Other Title
  • Phos-tag<sup>TM</sup> SDS–PAGEを用いたRabリン酸化の解析

Search this article

Abstract

<p>LRRK2 (leucine-rich repeat kinase 2) is one of the causative gene products for familial Parkinson’s disease (PD), harboring a Ser/Thr kinase domain with unknown functions. Recently we identified several small GTPase Rab proteins including Rab10 as physiological substrates of LRRK2, and we found that LRRK2 mutations linked with PD abnormally increase the Rab10 phosphorylation in cells and tissues. We utilized Phos-tag SDS–PAGE for detecting the phosphorylation of endogenous Rab10. On Phos-tag SDS–PAGE gels, one can detect protein phosphorylation as a bandshift, which enabled us to detect the endogenous levels of Rab10 phosphorylation without generating phospho-specific antibodies. In this review, we summarized the recent Phos-tag analysis on Rab10 phosphorylation by LRRK2.</p>

Journal

References(12)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top