Molecular characterization of multi-drug resistant coagulase negative cocci in non-hospital environment
-
- Nwibo Daniel Don
- Teikyo University Institute of Medical Mycology Laboratory of Immunology and Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
-
- Panthee Suresh
- Teikyo University Institute of Medical Mycology
-
- Hamamoto Hiroshi
- Teikyo University Institute of Medical Mycology
-
- Sekimizu Kazuhisa
- Teikyo University Institute of Medical Mycology Genome Pharmaceuticals Institute
この論文をさがす
説明
<p>Antibiotic resistance crisis occasioned by sporadic appearance of multi-drug resistance (MDR) in human pathogens to clinically applied antimicrobials is a serious threat to global health. In this study, we investigated the drug resistant phenotype of Gram-positive cocci isolates from environment. Staphylococcus capitis and Staphylococcus haemolyticus colonies were isolated on mannitol-salt agar plates supplemented with tetracycline. Antibiotic susceptibility profile of the isolates via minimum inhibitory concentration (MIC) determination was examined. Isolates showed decreased sensitivity to clinically applied antimicrobial agents: tetracycline, kanamycin, erythromycin, norfloxacin, teicoplanin, and ampicillin. Genomic analysis demonstrated the presence of multiple antibiotic resistant genes in these bacteria, suggesting the origin of the multiple antimicrobials resistant phenotype. Tetracycline resistance of these isolates was transduced to Staphylococcus aureus-RN4220 strain. These findings indicate the presence of multiple antimicrobials resistant S. capitis and S. haemolyticus strain in a non-hospital setting. Moreover, the presence of plethora of genes responsible for MDR suggest that these strains could present potential threat to human health by serving as reservoir for lateral transference of antimicrobial resistance conferring foreign genetic elements to other clinically relevant pathogens.</p>
収録刊行物
-
- Drug Discoveries & Therapeutics
-
Drug Discoveries & Therapeutics 13 (3), 145-149, 2019-06-30
特定非営利活動法人 バイオ＆ソーシャル・サイエンス推進国際研究交流会
