Structome of Yeast Cells Determined by Freeze-Substitution and Serial Ultrathin Sectioning Electron Microscopy
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- Yamaguchi Masashi
- 千葉大学・真菌医学研究センター
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- Okada Hitoshi
- 千葉大学・真菌医学研究センター 認定NPO法人 綜合画像研究支援
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- Namiki Yuichi
- 千葉大学・真菌医学研究センター
Bibliographic Information
- Other Title
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- 凍結置換法と連続切片法による酵母のストラクトーム解析
- トウケツ チカンホウ ト レンゾク セッペンホウ ニ ヨル コウボ ノ ストラクトーム カイセキ
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Abstract
<p>True understanding of cell structure comes only from quantitative three-dimensional structural analysis. The term “structome”, coined by combining “structure” and “-ome”, is defined as quantitative three-dimensional structural information of whole cells at the electron microscopic level. We report here, for the first time, the structome of the cells of yeast Exophiala dermatitidis in the G1 phase determined using freeze-substitution and serial ultrathin sectioning electron microscopy. The cell wall and the nucleus occupied ~22% and ~7% of the cell volume respectively. There were 17-52 mitochondria per cell, occupying 7-12% of the cell volume. Five to ten endoplasmic reticula were present in a cell, occupying ~0.2% of the cell volume: they did not form a network in the cell. There were 1-4 vacuoles in a cell, occupying 4-10% of the cell volume. The Golgi apparatus, spindle pole body, autophagosomes, multivesicular bodies, lipid bodies, microtubules and microfilaments occupied ~1% of the cell volume in total. About 200,000 ribosome particles and 1,000 glycogen granules were present per cell. The cytosol occupied 43-53% of the cell volume. The membranes of cells of this yeast could be classified into three groups by their appearance and thickness.</p>
Journal
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- KENBIKYO
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KENBIKYO 45 (4), 212-217, 2010-12-30
The Japanese Society of Microscopy
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Details 詳細情報について
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- CRID
- 1390846609797455872
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- NII Article ID
- 10031144705
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- NII Book ID
- AA11917781
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- ISSN
- 24342386
- 13490958
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- NDL BIB ID
- 10966765
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed