Application of Hydrogen Peroxide-Melanin Bleaching and Fluorescent Nuclear Staining for Whole-Body Clearing and Imaging in Fish

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  • Futami Kunihiko
    Department of Marine Biosciences, Tokyo University of Marine Science and Technology
  • Furukawa Oto
    Department of Marine Biosciences, Tokyo University of Marine Science and Technology
  • Maita Masashi
    Department of Marine Biosciences, Tokyo University of Marine Science and Technology
  • Katagiri Takayuki
    Department of Marine Biosciences, Tokyo University of Marine Science and Technology

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Other Title
  • メラニン色素を持つ魚の全身透明化と内部構造可視化のための蛍光核染色

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<p>Recently, our lab reported that CUBIC, a tissue-clearing technique, could help reveal the initial route of infection, its spread, and the localization of pathogens in infected goldfish Carassius auratus. ​However, this technique, in its original form, failed to clear melanin pigmented parts of the fish. ​We show here that bleaching with H2O2 clears melanin pigments of Black Moor goldfish without causing severe histological damage. ​Furthermore, nuclear staining with PI helps visualize the internal structures of cleared fish in a dark field. ​Since bleaching does not significantly quench the fluorescence of GFP, it may be applied to the in vivo imaging of GFP.</p>

Journal

  • Fish Pathology

    Fish Pathology 54 (4), 101-103, 2020-01-15

    The Japanese Society of Fish Pathology

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