Macromolecular Crystallography Using Long Wavelength X-ray

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  • MATSUGAKI Naohiro
    Institute of Materials Structure Science, High Energy Accelerator Research Organization
  • YAMADA Yusuke
    Institute of Materials Structure Science, High Energy Accelerator Research Organization
  • HIKITA Masahide
    Institute of Materials Structure Science, High Energy Accelerator Research Organization
  • HIRAKI Masahiko
    Institute of Particle and Nuclear Studies, High Energy Accelerator Research Organization
  • SENDA Miki
    Institute of Materials Structure Science, High Energy Accelerator Research Organization
  • SENDA Toshiya
    Institute of Materials Structure Science, High Energy Accelerator Research Organization

Bibliographic Information

Other Title
  • 長波長X線を利用したタンパク質結晶構造解析
  • 世界の放射光施設を使ってみよう(4)長波長X線を利用したタンパク質結晶構造解析
  • セカイ ノ ホウシャコウ シセツ オ ツカッテ ミヨウ(4)チョウハチョウ Xセン オ リヨウ シタ タンパクシツ ケッショウ コウゾウ カイセキ

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Abstract

<p>Applying long wavelength X-ray （ca. longer than 2 Å） in macromolecular crystallography has been limited due to the lack of dedicated measurement environment. Photon Factory BL-1A, as an MX beamline optimized for diffraction experiment using the wavelength from 2.7 to 3.3 Å, offers the opportunity to routinely perform data collection for Native SAD phasing or light atoms identification with much sophisticated manner. Sample optimization is another key for successful experiment at the wavelength where severe absorption is inevitable. We describe how the difficulties in use of long wavelength X-ray were overcome at the beamline and the auxiliary facility, as well as the current results of Native SAD phasing.</p>

Journal

  • Nihon Kessho Gakkaishi

    Nihon Kessho Gakkaishi 62 (1), 56-61, 2020-02-29

    The Crystallographic Society of Japan

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